Reproducibility

Reproducibility is crucial in chromatography, signifying the consistency and reliability of results over multiple tests.

High reproducibility ensures stable retention times and peak areas, which is vital for quantitative analysis and data integrity.

Achieving this depends on key factors like stable column performance, precise control of the mobile phase, proper system maintenance, and consistent sample preparation. It’s measured using the relative standard deviation (RSD) of results, with values typically expected to be below 1% for well-optimized systems.

In regulated industries, reproducibility is a strict regulatory requirement, emphasizing the importance of quality columns and robust standard operating procedures (SOPs).